Physicochemical properties of polymers: An important system to overcome the cell barriers in gene transfection
Identifieur interne : 000F33 ( Main/Exploration ); précédent : 000F32; suivant : 000F34Physicochemical properties of polymers: An important system to overcome the cell barriers in gene transfection
Auteurs : Ali Namvar [Iran] ; Azam Bolhassani [Iran] ; Niloofardokht Khairkhah [Iran] ; Fatemeh Motevalli [Iran] ; David CaseSource :
- Biopolymers [ 0006-3525 ] ; 2015-07.
Abstract
Delivery of the macromolecules including DNA, miRNA, and antisense oligonucleotides is typically mediated by carriers due to the large size and negative charge. Different physical (e.g., gene gun or electroporation), and chemical (e.g., cationic polymer or lipid) vectors have been already used to improve the efficiency of gene transfer. Polymer‐based DNA delivery systems have attracted special interest, in particular via intravenous injection with many intra‐ and extracellular barriers. The recent progress has shown that stimuli‐responsive polymers entitled as multifunctional nucleic acid vehicles can act to target specific cells. These nonviral carriers are classified by the type of stimulus including reduction potential, pH, and temperature. Generally, the physicochemical characterization of DNA‐polymer complexes is critical to enhance the transfection potency via protection of DNA from nuclease digestion, endosomal escape, and nuclear localization. The successful clinical applications will depend on an exact insight of barriers in gene delivery and development of carriers overcoming these barriers. Consequently, improvement of novel cationic polymers with low toxicity and effective for biomedical use has attracted a great attention in gene therapy. This article summarizes the main physicochemical and biological properties of polyplexes describing their gene transfection behavior, in vitro and in vivo. In this line, the relative efficiencies of various cationic polymers are compared. © 2015 Wiley Periodicals, Inc. Biopolymers 103: 363–375, 2015.
Url:
DOI: 10.1002/bip.22638
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001A38
- to stream Istex, to step Curation: 001A38
- to stream Istex, to step Checkpoint: 000078
- to stream Main, to step Merge: 000F34
- to stream Main, to step Curation: 000F33
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Physicochemical properties of polymers: An important system to overcome the cell barriers in gene transfection</title><author><name sortKey="Namvar, Ali" sort="Namvar, Ali" uniqKey="Namvar A" first="Ali" last="Namvar">Ali Namvar</name></author><author><name sortKey="Bolhassani, Azam" sort="Bolhassani, Azam" uniqKey="Bolhassani A" first="Azam" last="Bolhassani">Azam Bolhassani</name></author><author><name sortKey="Khairkhah, Niloofardokht" sort="Khairkhah, Niloofardokht" uniqKey="Khairkhah N" first="Niloofardokht" last="Khairkhah">Niloofardokht Khairkhah</name></author><author><name sortKey="Motevalli, Fatemeh" sort="Motevalli, Fatemeh" uniqKey="Motevalli F" first="Fatemeh" last="Motevalli">Fatemeh Motevalli</name></author><author wicri:is="90%"><name sortKey="Case, David" sort="Case, David" uniqKey="Case D" first="David" last="Case">David Case</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:CB561CDF12B91E958DEC72AA71EB35E79A5F14DA</idno><date when="2015" year="2015">2015</date><idno type="doi">10.1002/bip.22638</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-X67MGN0F-F/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001A38</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001A38</idno><idno type="wicri:Area/Istex/Curation">001A38</idno><idno type="wicri:Area/Istex/Checkpoint">000078</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000078</idno><idno type="wicri:doubleKey">0006-3525:2015:Namvar A:physicochemical:properties:of</idno><idno type="wicri:Area/Main/Merge">000F34</idno><idno type="wicri:Area/Main/Curation">000F33</idno><idno type="wicri:Area/Main/Exploration">000F33</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Physicochemical properties of polymers: An important system to overcome the cell barriers in gene transfection</title><author><name sortKey="Namvar, Ali" sort="Namvar, Ali" uniqKey="Namvar A" first="Ali" last="Namvar">Ali Namvar</name><affiliation wicri:level="1"><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Bolhassani, Azam" sort="Bolhassani, Azam" uniqKey="Bolhassani A" first="Azam" last="Bolhassani">Azam Bolhassani</name><affiliation wicri:level="1"><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation><affiliation></affiliation></author><author><name sortKey="Khairkhah, Niloofardokht" sort="Khairkhah, Niloofardokht" uniqKey="Khairkhah N" first="Niloofardokht" last="Khairkhah">Niloofardokht Khairkhah</name><affiliation wicri:level="1"><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author><name sortKey="Motevalli, Fatemeh" sort="Motevalli, Fatemeh" uniqKey="Motevalli F" first="Fatemeh" last="Motevalli">Fatemeh Motevalli</name><affiliation wicri:level="1"><country xml:lang="fr">Iran</country><wicri:regionArea>Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran</wicri:regionArea><wicri:noRegion>Tehran</wicri:noRegion></affiliation></author><author wicri:is="90%"><name sortKey="Case, David" sort="Case, David" uniqKey="Case D" first="David" last="Case">David Case</name></author></analytic><monogr></monogr><series><title level="j" type="main">Biopolymers</title><title level="j" type="alt">BIOPOLYMERS</title><idno type="ISSN">0006-3525</idno><idno type="eISSN">1097-0282</idno><imprint><biblScope unit="vol">103</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="363">363</biblScope><biblScope unit="page" to="375">375</biblScope><biblScope unit="page-count">13</biblScope><date type="published" when="2015-07">2015-07</date></imprint><idno type="ISSN">0006-3525</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0006-3525</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract">Delivery of the macromolecules including DNA, miRNA, and antisense oligonucleotides is typically mediated by carriers due to the large size and negative charge. Different physical (e.g., gene gun or electroporation), and chemical (e.g., cationic polymer or lipid) vectors have been already used to improve the efficiency of gene transfer. Polymer‐based DNA delivery systems have attracted special interest, in particular via intravenous injection with many intra‐ and extracellular barriers. The recent progress has shown that stimuli‐responsive polymers entitled as multifunctional nucleic acid vehicles can act to target specific cells. These nonviral carriers are classified by the type of stimulus including reduction potential, pH, and temperature. Generally, the physicochemical characterization of DNA‐polymer complexes is critical to enhance the transfection potency via protection of DNA from nuclease digestion, endosomal escape, and nuclear localization. The successful clinical applications will depend on an exact insight of barriers in gene delivery and development of carriers overcoming these barriers. Consequently, improvement of novel cationic polymers with low toxicity and effective for biomedical use has attracted a great attention in gene therapy. This article summarizes the main physicochemical and biological properties of polyplexes describing their gene transfection behavior, in vitro and in vivo. In this line, the relative efficiencies of various cationic polymers are compared. © 2015 Wiley Periodicals, Inc. Biopolymers 103: 363–375, 2015.</div></front></TEI><affiliations><list><country><li>Iran</li></country></list><tree><noCountry><name sortKey="Case, David" sort="Case, David" uniqKey="Case D" first="David" last="Case">David Case</name></noCountry><country name="Iran"><noRegion><name sortKey="Namvar, Ali" sort="Namvar, Ali" uniqKey="Namvar A" first="Ali" last="Namvar">Ali Namvar</name></noRegion><name sortKey="Bolhassani, Azam" sort="Bolhassani, Azam" uniqKey="Bolhassani A" first="Azam" last="Bolhassani">Azam Bolhassani</name><name sortKey="Khairkhah, Niloofardokht" sort="Khairkhah, Niloofardokht" uniqKey="Khairkhah N" first="Niloofardokht" last="Khairkhah">Niloofardokht Khairkhah</name><name sortKey="Motevalli, Fatemeh" sort="Motevalli, Fatemeh" uniqKey="Motevalli F" first="Fatemeh" last="Motevalli">Fatemeh Motevalli</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000F33 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000F33 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:CB561CDF12B91E958DEC72AA71EB35E79A5F14DA
|texte= Physicochemical properties of polymers: An important system to overcome the cell barriers in gene transfection
}}
| This area was generated with Dilib version V0.6.33. |  |